ABSTRACT
Objective To investigate the effects of respiratory training on oral feeding ability, feeding response, transition time, hospitalization time, gastric tube indwelling time and recovery time to birth weight in premature infants. Methods The Neonatal Intensive Care Unit(NICU) preterm infants who were in accordance with the inclusion criteria were divided into the observation group and the control group according to the random number method. The observation group was 20 cases, the control group was 21 cases. The control group implemented NICU routine nursing, including oral nursing, umbilical nursing, body position nursing. The observation group received respiratory training exercises on the basis of the control group, and the oral feeding ability of the two groups of premature infants was evaluated and recorded after intervention at the beginning of 0, 5, 10, 15 d.The feeding reaction, transition time, the hospitalization time and gastric tube indwelling time were recorded in two groups of preterm infants. Results The non-nutritive sucking scores after intervention at the beginning of 0, 5, 10, 15 d were (31.10±5.57), (37.50±4.86), (53.20±7.60), (74.90±4.77) points in the observation group . The non-nutritive sucking scores after intervention at the beginning of 0, 5, 10, 15 d were (31.91±5.56), (33.36±6.17), (45.54±5.39), (62.82±6.78) points in the control group. There was no significant difference after intervention at the beginning of 0, 5 d between the two groups (t=-0.333,1.694, all P>0.05), but had the significant difference after intervention at the beginning of 10 ,15 d (t=2.683, 4.676, all P <0.05). The transition time,gastric tube indwelling time and recovery time to birth weight in premature infants was (7.15±1.72), (11.00±2.37), (7.86±1.43) d in the observation group and (10.33±2.44), (16.14±2.29), (9.97±1.69) d in the control group, and there were significant differences between the two groups(t=-4.807, 3.335,-9.905, P <0.05 ). There was no significant difference in the hospitalization time between the two groups(P>0.05). Conclusions Breathing exercises can improve the oral feeding ability and improve the feeding effect of premature infants.
ABSTRACT
Objective:To study the preventive and therapeutic effects of silk fibroin peptides (SFP) on acute alcoholism in mice. Methods:In first experiment, the mice were randomly divided into four groups, and every group was treated by 56o alcohol (ethanol dosage 7.56 ml/kg bw) via i.g. Eighty mice were fed normal saline (NS) and different dosages of SFP (0.1, 0.5, 2.5 g/kg bw) 30 min later respectively, then the rate of ebriety and time of sobriety were determined. Another 72 mice were also divided into four groups and given NS and SFP similarly. The concentration of ethanol in serum was measured 1 h, 2 h and 4 h later respectively. In second experiment, the mice were also divided into four similar groups, but 56o alcohol was given at 6.16 ml/kg?bw via i.g. NS and SFP were given similarly, 0.5 h before alcohol. Two experiments were performed to observe the effect of SFP on prevention of temulence. Results:The time of sobriety and concentration of ethanol of SFP groups fed 0.5 and 2.5g/kg bw were lower significantly than those of NS group (P
ABSTRACT
Objective: To screen out DNA binding proteins specifically recognizing CpG immunostimulatory sequence (ISS) for further investigating the molecular mechanisms of ISS. Methods: Yeast one hybrid system was adapted in screening a human bone marrow cDNA library using 4 copies of ISS as bait. The ISS binding activity of the positive clone was confirmed by electrophoretic mobility shift assay (EMSA). Results: Four dual positive colonies were obtained, two of them encoded proteins with unknown functions. The other 2 encoded light chains of immunoglobulin with amino sequences homology to anti DNA Ab and HBsAb respectively. EMSA showed HBsAb specifically bound to CpG ODN at pH6.4 and pH 5.8. Conclusion: HBsAb may have ISS specific DNA binding activity.